Tag
SpyTag

Part:BBa_K3183013:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-16)


SpyTag, Codon-Optimized for L. reuteri


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RF10, 12, 21, 23, 25, and 1000 compatible; Codon-optimzed for L. reuteri using Kazusa codon usage database tool.

Source

Protein Sequence from Zakeri et al, 2012. Codon-optimized using L. reuteri data from http://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1598

References

Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. (2012). Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 20;109(12)